where, release of BSA on HA microsphere initially exhibits a linear increase behavior with time, but nearly come to an end after 48 h. A similar study has also been illustrated by Fu et al. illustrates the feasibility of fast release of BSA from hydroxyapatite microspheres upto 70 wt% within 0.5 h in phosphate buffer solution at 37 ☌.

In another study, surface of nanoscale particle influences osteoblast and mesenchymal stem cell growth and their proliferation. Recently, protein adsorption on hydroxyapatite surface study significantly enhances and reports on cell adhesion, viability and chondrogenesis on the nano-biointerface. Hence, prospective development of nanobiomaterials for bone tissue replacement, coating on medical prosthesis and tissue engineering demands an understanding the interaction phenomenon within synthetic biomaterial and protein which strictly depends on the morphology and crystal structure of biomaterials.

However, the biomaterial during in vivo implantation or tissue engineering has essential need for the immediate adherence protein molecules from blood and body fluids to continue cellular response, cell spreading, migration and proliferation through protein coated layer. Nano size dimension of HA with high surface to volume ratio and reactivity promotes as potential biomaterial for different area such as bone growth factor, protein delivery media, bone implant and coating on metal implant. Biological properties like bioresorption, bioactivity and biocompatibility of hydroxyapatite (HA) facilitate as an ideal material for hard tissue repair.

Ha swain